Thermo Scientific™

Canine Genotypes Panel 1.1 Kit

Thermo Scientific Canine Genotypes Panel 1.1 is a reagents kit for canine parentage testings and identification using Short Tandem Repeat자세히 알아보기

Thermo Scientific Canine Genotypes Panel 1.1 is a reagents kit for canine parentage testings and identification using Short Tandem Repeat (STR) DNA loci. Due to their high level of informativeness and Mendelian inheritance, microsatellites have become the markers of choice for parentage testing, identification and forensics.

The Canine Genotypes Panel 1.1 provides all the necessary reagents for amplification of the 19 microsatellite loci. The kit contains master mix and primer mix allows co-amplification of the microsatellites in a single multiplex PCR reaction. Canine control DNA is provided to verify acceptable PCR and electrophoresis conditions. Reagents and protocols of the Canine Genotypes Panel 1.1 kit have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of plus-A peaks or split peaks typically interpreted when using e.g. a conventional Taq DNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the plus-A peaks. One primer from each primer pair is end-labelled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated capillary electrophoresis instrument.

Canine Genotypes Panel 1.1 encompasses the following 19 loci: AHTk211, CXX279, REN169O18, INU055, REN54P11, INRA21, AHT137, REN169D01, AHTh260, AHTk253, INU005, INU030, FH2848, AHT121, FH2054, REN162C04, AHTh171, REN247M23 and Amelogenin (sex determining locus). These markers are included in the core panel of loci recommended by the Applied Genetics Committee of Companying Animals of the International Society for Animal Genetics (ISAG).

Highlights

• Single multiplex PCR of 19 STR loci
• Master mix and Primer mix provided for easy pipetting
• Control DNA provided for verification of PCR and electrophoresis conditions
• Allele calls represents true alleles (no plus-A peaks)

사양

검출 방법Capillary Electrophoresis

용도(애플리케이션)PCR

반응 수100

제품 유형Canine Genotypes Panel 1.1 Kit

타겟Canine Genotyping and Parentage Testing

기술STR (Short Tandem Repeat) Analysis

Unit SizeEach