Thermo Fisher Scientific ZO-1 Recombinant Mouse Monoclonal Antibody (ZO1-1A12), Alexa Fluor Plus 647

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunocytochemistry (ICC/IF)

1:2,000-1:4,000

-

Flow Cytometry (Flow)

0.063-0.5 µg/test

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

ZO1-1A12

Immunogen

Human recombinant ZO-1 fusion protein encompassing amino acids 334-634 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ZO-1,uniProtId:Q07157-1,ncbiNodeId:9606,antigenRange:334-634,antigenLength:1748,antigenImageFileName:740002MP647_ZO-1_Q07157-1_House_mouse.svg,antigenImageFileNamePDP:740002MP647_ZO-1_Q07157-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647

View additional formats

• Unconjugated
• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Request custom conjugation

Excitation/Emission Max

658/675 nm View spectra

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A/G

Storage buffer

proprietary buffer, pH 6.5

Contains

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_3093163

Product Specific Information

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Applications Tested: This ZO-1 antibody has been tested by immunohistochemistry of mouse testis tissue and immunocytochemistry and flow cytometric analysis of Caco-2 cells. This may be used at 0.063-0.5 µg per test for flow cytometry. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.