Thermo Fisher Scientific ZO-1 Recombinant Mouse Monoclonal Antibody (ZO1-1A12), Alexa Fluor Plus 647
Alexa Fluor Plus 647 형광으로 표지된 ZO-1 재조합 마우스 단클론 항체. IHC, ICC/IF, Flow Cytometry에 사용 가능. 높은 신호 대 잡음비와 일관된 로트 간 품질을 제공하며, 세포 간 타이트 접합 연구에 적합.
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Thermo Fisher Scientific ZO-1 Recombinant Mouse Monoclonal Antibody (ZO1-1A12), Alexa Fluor Plus 647
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:2,000–1:4,000 |
| Flow Cytometry (Flow) | 0.063–0.5 µg/test |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ZO1-1A12 |
| Immunogen | Human recombinant ZO-1 fusion protein (amino acids 334–634) |
| Conjugate | Alexa Fluor™ Plus 647 |
| Excitation/Emission Max | 658/675 nm |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | Proprietary buffer, pH 6.5 |
| Contains | 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_3093163 |
Additional Formats
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies utilize proprietary dye chemistry for enhanced fluorescence intensity and improved signal-to-noise ratio compared to standard Alexa Fluor conjugates. These recombinant antibodies ensure high specificity and lot-to-lot consistency.
Usage Note:
Before use, centrifuge the conjugate solution briefly and use only the supernatant to minimize nonspecific background staining caused by protein aggregates.
Applications Tested:
This ZO-1 antibody has been validated in:
- Immunohistochemistry of mouse testis tissue
- Immunocytochemistry and flow cytometry analysis of Caco-2 cells
Recommended concentration for flow cytometry: 0.063–0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test).
Careful titration is recommended for optimal performance.
Excitation: 658 nm
Emission: 675 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
ZO-1 forms complexes with ZO-2 or ZO-3 and associates with claudin, occludin, and F-actin at tight junctions, linking the actin cytoskeleton to the junctional complex. ZO-1 expression is reduced in many breast cancer lines. It may play a role in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms are known.
Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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