Invitrogen™

2-YT Broth

2-YT Broth is a nutrient-rich microbial growth medium used for cultivation of E. coli and growth of hosts for replication자세히 알아보기

2-YT Broth is a nutrient-rich microbial growth medium used for cultivation of E. coli and growth of hosts for replication of M13 vectors. The 2-YT formulation is similar to Lennox LB medium, but contains double the concentration of yeast extract and a 60% higher concentration of peptone. 2-YT medium allows cells to reach higher density and increase recombinant protein production compared to LB media formulations.

2-YT Broth formulation
Formulation per liter: 16 g SELECT Peptone 140 (pancreatic digest of casein), 10 g SELECT Yeast Extract, and 5 g NaCl.
Note: To prepare 1 L of 2-YT medium, weigh 31 g of powder and add distilled water to 1 L. Adjust the pH value to 7.0 with 5 N NaOH and sterilize by autoclaving for 20 minutes at 121°C.

Primary applications
• Growth of hosts for replication of M13 vectors
• Recombinant protein expression
• Achieve higher plasmid DNA yield

Find the media format that fits you needs
We offer a variety of ready-to-use media formulations including LB Broth, Terrific Broth and S.O.C Recovery Medium.
Explore our media formats for bacterial growth.

사양

종Escherichia coli

형식Bottle

미디어 유형2-YT Broth (Yeast Extract Tryptone)

준비 방법Autoclave

제품 유형Dehydrated Culture Media

타겟 유기체 분류Coliforms

수량500 g

배송 조건Room Temperature

Unit SizeEach