Gibco™

MEM, autoclavable, no glutamine, powder

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used자세히 알아보기

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With	Without
• Phenol Red	• L-glutamine
• Earle`s salts	• HEPES
	• Sodium Bicarbonate

The complete formulation is available.

Gibco MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgows MEM, MEM α, DMEM, and Temins Modification. To allow autoclaving this medium, choline chloride is substituted with choline bitartrate, phenol red is reduced, and succinic acid is added to prevent precipitation at low pH. This MEM formulation contains Earles salts for use in a CO2 incubator. This product is made with Earles salts.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

This autoclavable form of Gibco™ cell culture medium requires pH adjustment to 4.1–4.2 before autoclaving, following by sodium bicarbonate and L-glutamine supplementation and final pH adjustment to 7.2–7.4 (see protocol for details).

사양

세포주HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts

세포 유형Primary Rat Astrocytes

분류Animal Origin-free

형태Powder

제품 유형MEM (Minimum Essential Medium)

Serum LevelStandard Serum Supplementation

유통 기한36 Months From Date of Manufacture

멸균Sterile-filtered

첨가제 포함Phenol Red, Earle`s Balanced Salt Solution (EBSS)

첨가제 없음No HEPES, No Sodium Bicarbonate

제조 품질cGMP-compliant under the ISO 13485 standard

제품라인Gibco™

수량10 L

배송 조건Room Temperature

Unit SizeEach