Thermo Scientific™

T4 Polynucleotide Kinase (10 U/μL)

Thermo Scientific™ T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5`-OH group of자세히 알아보기

Thermo Scientific™ T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides, or nucleoside 3-monophosphates (forward reaction). The reaction is reversible. In the presence of ADP, T4 Polynucleotide Kinase exhibits 5-phosphatase activity and catalyzes the exchange of phosphate groups between 5-P-oligo-polynucleotides and ATP (exchange reaction). The enzyme is also a 3`-phosphatase.

Highlights
• Active in Thermo Scientific restriction enzyme, RT, and T4 DNA ligase buffers

Applications
• Labeling 5-termini of nucleic acids to be used as: --Probes for hybridization --Probes for transcript mapping --Markers for gel electrophoresis --Primers for DNA sequencing --Primers for PCR • 5-phosphorylation of oligonucleotides, PCR products, other DNA or RNA prior to ligation
• Phosphorylation of PCR primers
• Detection of DNA modification by the [32P]-postlabeling assay
• Removal of 3`-phosphate groups

Notes
• The 5`-termini of nucleic acids can be labeled by either the forward or the exchange reaction.
• Polyethylene glycol (PEG) and spermidine improve the rate and efficiency of the phosphorylation reaction. PEG is used in the exchange reaction mixture.
• Since T4 Polynucleotide Kinase is inhibited by ammonium ions, use sodium acetate to precipitate DNA prior to phosphorylation.

사양

농도10 U/μL

효소T4 Polynucleotide Kinase

함께 사용가능한 버퍼10X Reaction Buffer

수량500 units

제품 유형T4 Polynucleotide Kinase

Unit SizeEach