Thermo Fisher Scientific Active Caspase 3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50-1:500

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

10-20 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

10-20 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Product Specifications

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2243982

Product Specific Information

Positive controls of rat brain or foetus, or mouse foetus suggested.

Target Information

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer`s disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.