Thermo Fisher Scientific TFAM Polyclonal Antibody
TFAM 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC, Flow 등 다양한 응용에 적합합니다. 인간 및 마우스 반응성을 가지며, 단백질 A 및 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 3 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | - |
| Immunohistochemistry - Free Floating (IHC (Free)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:10–1:50 | View 2 publications |
| Flow Cytometry (Flow) | 1:10–1:50 | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 216–246 amino acids from the C-terminal region of human TFAM |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.38 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2541276 |
Target Information
Mitochondrial transcription factor A (TFAM) is a crucial nuclear-encoded protein transported to mitochondria, where it maintains and regulates mitochondrial DNA (mtDNA) replication and transcription. TFAM interacts with LC3 via its LC3 interacting region (LIR) motif, playing an essential role in autophagy and inflammation control by eliminating leaked mtDNA. TFAM levels influence mitochondrial function; high expression can repress mtDNA transcription in skeletal muscle, while balanced TFAM-to-mtDNA ratios preserve oxidative phosphorylation capacity in cardiac tissue. Dysregulation of TFAM is associated with neurodegeneration and diseases related to mtDNA stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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