Invitrogen™

Platinum™ SYBR™ Green qPCR SuperMix-UDG w/ROX

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we`ve taken the best of Platinum SYBR Green qPCR SuperMix-UDG and added additional capabilities for your gene expression analysis.

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qPCR SuperMixes with ROX for ABI instruments are ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or plasmid DNA. ROX reference dye is included in the mix for maximum convenience. These qPCR SuperMixes include:

–Platinum™ Taq DNA Polymerase: activates faster and more completely than other hot-start enzymes to deliver more sensitive amplification (Figure 1)

–Uracil-DNA Glycosylase (UDG): prevents carryover contamination in conjunction with dUTP (included) for improved specificity
–ROX reference dye premixed at optimal concentration for ABI instruments

qPCR SuperMixes with ROX are available in two formats:

–Platinum™ SYBR™ Green qPCR SuperMix-UDG with ROX provides a sensitive, cost-effective system for real-time PCR detection with SYBR™ Green I dye (Figure 2)
–Platinum™ qPCR SuperMix-UDG with ROX offers superior performance with either fluorogenic primers (e.g., LUX™ Primers) or probe-based detection systems (e.g., TaqMan™)

Contents and Storage:
Platinum™ SYBR™ Green qPCR SuperMix-UDG with ROX includes a 2X SuperMix with SYBR™ Green I and ROX Reference Dyes, and 50 mM MgCl2. Platinum™ qPCR SuperMix-UDG with ROX includes a 2X SuperMix with ROX Reference Dye, and 50 mM MgCl2. Store all components at -20°C. Guaranteed stable for 6 months when properly stored.

사양

검출 방법SYBR

GC-Rich PCR PerformanceHigh

PCR 방법qPCR

중합효소Taq DNA Polymerase

반응 속도Standard

용도(장비)7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System

제품라인Platinum™, SYBR™

제품 유형Real Time PCR SYBR Master Mix

수량100 reactions

배송 조건Dry Ice

충분100 Reactions

용도(애플리케이션)Gene Expression

호환성ABI PRISM™ 7000, ABI PRISM™ 7700, ABI PRISM™ 7900HT, ABI PRISM™ 7900HT Fast, ABI 7300 Real-Time PCR System, ABI GeneAmp™ 5700

농도2X

반응 수100 Reactions

샘플 종류DNA (Genomic), cDNA

Unit Size100 reactions