Thermo Fisher Scientific ZSCAN4 Monoclonal Antibody (OTI3D1), TrueMAB
ZSCAN4 단백질을 인식하는 마우스 단클론 항체로, Western blot에 최적화됨. 인간 유래 단백질(aa 164-433)을 면역원으로 제작. 동결건조 형태로 제공되며, 재구성 시 1 mg/mL 농도로 사용 가능. 줄기세포 및 배아 발달 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3D1 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 164–433 of human ZSCAN4 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
- For conjugation experiments, perform an additional desalting step (e.g., using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Zygotic genome activation (ZGA) is a critical transition from maternal to embryonic genetic control, occurring mainly at the late 2-cell stage.
ZSCAN4 encodes a SCAN domain and four zinc finger domains, expressed in late 2-cell embryos and embryonic stem cells.
Loss-of-function studies using siRNA show delayed progression from 2-cell to 4-cell stages and impaired embryo development.
ZSCAN4 is involved in telomere maintenance and genomic stability in embryonic stem cells, indicating its essential role in maintaining pluripotency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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