Thermo Fisher Scientific ASH2L Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.2–1 µg/mL

Immunohistochemistry (IHC)

• Tested Dilution: 5 µg/mL

Product Specifications

Product Specific Information

Peptide sequence:
AAGSSGKGRG AKRKQQDGGT TGTTKKARSD PLFSAQRLPP HGYPLEHPFN

Sequence homology:
Cow: 86%
Dog: 100%
Guinea Pig: 100%
Horse: 100%
Human: 100%
Mouse: 100%
Rabbit: 100%
Rat: 100%

Target Information

The human ASH2L gene encodes a 628 amino acid protein known as ASH2L1 (isoform 1), which contains a nuclear localization signal and PHD finger motif, suggesting a role as a transcription regulator.
Predicted molecular weight: 68.7 kDa
Alternative splicing may produce isoforms at 60 kDa and 56.5 kDa.

Human ASH2L proteins are 60% homologous to Drosophila ash2, which positively regulates gene expression during early development and contains similar zinc finger motifs. Homology to mouse ASH2L is 90%.

ASH2L is predominantly expressed in adult heart and testis, and fetal lung and liver. Expression is low in adult lung, liver, kidney, prostate, and peripheral leukocytes, as well as in leukemia cell lines with erythroid and megakaryocytic potential (e.g., K562, Hel, Dami). Differentiation inducers such as phorbol ester and hemin alter expression patterns, suggesting that ASH2L plays a role in hematopoiesis and may be associated with particular types of leukemia. Additionally, ASH2L has been suggested to interact with beta-tubulin and beta-catenin.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.