Applied Biosystems™

CLA IdentiFiler™ Plus PCR Amplification Kit

The CLA (Cell Line Authentication) IdentiFiler Plus PCR Amplification Kit simplifies the amplification step during authentication of cell lines. It자세히 알아보기

The CLA (Cell Line Authentication) IdentiFiler Plus PCR Amplification Kit simplifies the amplification step during authentication of cell lines. It includes the same primers and allelic ladder as the widely used AmpFLSTR Identifiler Plus kit and harnesses next-generation PCR amplification technology to help provide enhanced sensitivity, a cleaner baseline, improved performance with mixtures, and the ability to overcome high levels of PCR inhibition. The kit includes GeneScan 600 LIZ Size Standard v2.0, a five-dye-labeled, high-density size standard for the reproducible sizing of fragment analysis data.

Features of the CLA IdentiFiler Plus PCR Amplification Kit include:
• 5-dye, 16-locus STR kit compatible with all cell line databasing loci standards
• Improved version of legacy AmpFLSTR Identifiler Plus kit
• Size standard included for ease of purchase
• Compatible with SeqStudio, 3730/xL, and 3500/xL genetic analyzers

Cell line authentication using short tandem repeat (STR) analysis kits can be used both for human sample authentication (HSA) and mixed sample analysis (MSA). In HSA, there is a need to verify the DNA profile of the sample to check for sample cross-contamination or sample mix-up, or to perform a quality check. For MSA there is a need to deconvolute the presence of multiple contributors in a sample (chimerism) through identification of multiple DNA genotypes in the sample.

사양

검출 방법Gel Electrophoresis

형태Liquid

형식Kit

GC-Rich PCR PerformanceHigh

PCR 방법End-point PCR

중합효소AmpliTaq Gold™ DNA Polymerase

반응 속도60 min.

기술STR (Short Tandem Repeats) Analysis

용도(장비)SeqStudio Flex™ 3500, SeqStudio™ 3730xl Genetic Analyzers

키트 유형Multicomponent

라벨 또는 염료LIZ, FAM, NED, PET, VIC

수량200 x 25 μL Reactions

종Human

타겟 유기체 분류Human cell lines

용도(애플리케이션)Genotyping, Cell Analysis

정제 타겟Cell DNA

반응 형식Tube

샘플 종류Genomic DNA

시작 물질Cell Line Culture

Unit SizeEach