Thermo Fisher Scientific PFKL/Fructose 6 Phosphate Kinase Polyclonal Antibody
PFKL/Fructose 6 Phosphate Kinase 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, WB, IHC, ICC, ELISA 등 다양한 실험에 사용 가능. Human, Mouse, Rat 시료에 반응하며 Protein A로 정제된 고품질 항체.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human Fructose 6 Phosphate Kinase (amino acids 701–780) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Phosphofructokinase (PFK) is a major regulatory enzyme involved in sugar metabolism and energy production in all cells. In humans, three structural loci control PFK subunits: M (muscle), L (liver), and P (platelet). These subunits form various tetramers, with muscle and liver PFKs being homotetramers (M₄ and L₄). Red blood cells express all five isozymes (M₄, M₃L, M₂L₂, ML₃, L₄). PFK is allosterically activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. It catalyzes the key regulatory step in glycolysis. PFK deficiency can cause episodic illness with dark-colored urine following strenuous exercise, and elevated PFK levels are observed in individuals with Down syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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