Thermo Fisher Scientific KLRB1 Monoclonal Antibody (10/78), APC

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.1-0.25 µg / 1x10^6 cells in 100 µL

View 2 publications 2 publications

Product Specifications

Published species

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

10/78

Immunogen

Rat NKR-P1A.

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 µg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_10376034

Product Specific Information

This antibody reacts with rat NKR-PIA, which is expressed by NK cells and NKT cells. Every lot is tested by flow cytometry using freshly harvested rat splenocytes.

Use dim light during handling, incubation with cells and prior to analysis. It is recommended that cells be analyzed within 18 hours of staining. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted.

Target Information

KLRB1 (Killer cell lectin like receptor subfamily B, member 1, CD-161) protein is classified as a type II membrane protein because it has an external C terminus. KLRB1 cell surface antigen is expressed by almost all NK cells and in a small subset of CD3+ve T cells. KLRB1 is a homodimeric cell surface protein, comprising two chains with molecular weights ranging from 40-44kDa. KLRB1 plays an inhibitory role on natural killer (NK) cells. Activation of KLRB1 leads to acid sphingomyelnase/SMPD1 stimulation and an increase in intracellular ceramide. Moreover, there is also an activation of AKT1/PKB and RPS6KA1/RSK1 kinase stimulation, and T cell proliferation by anti-CD3. KLRB1 acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope and to the N-acetyllactosamine epitope. KLRB1 also binds to CLEC2D/LLT1 as a ligand, and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.