Invitrogen™

BioPrime™ Array CGH Genomic Labeling System

The BioPrime™ Plus Array CGH Genomic Labeling Systems are a high-performance labeling kits that enable reproducible labeling of genomic DNA자세히 알아보기

The BioPrime™ Plus Array CGH Genomic Labeling Systems are a high-performance labeling kits that enable reproducible labeling of genomic DNA samples for array-based Comparative Genomic Hybridization (CGH). Available in both indirect and direct labeling formats, the BioPrime™ Plus Array CGH Genomic Labeling Systems provide a flexible solution to your genomic labeling needs. Using the
BioPrime™ Plus Array CGH Genomic Labeling Systems, you can expect:
––High yields of fluorescently labeled genomic DNA
–signal-to-noise ratios
–detection of gene copy number variations (Figure 1)

These systems combine a highly concentrated exo-Klenow fragment of DNA polymerase I and random primers to effectively label genomic targets for sensitive genomic profiling experiments. Exo-Klenow is a mutant of the large fragment of the DNA polymerase I holoenzyme that has both 5´ - 3´ and 3´ - 5´ exonuclease activity removed. The lack of exonuclease activity makes this enzyme ideal for use in random priming protocols, enabling robust yields and efficient incorporation of Alexa Fluor™ AHA fluorescent nucleotides. In the BioPrime™ Plus Array CGH Genomic Labeling Systems, random octamers anneal to template DNA, providing priming sites for the exo- Klenow enzyme. Alexa Fluor™ AHA modified nucleotides (direct labeling) or amino-allyl modified nucleotides (indirect labeling) are incorporated as the polymerase extends from the priming sites. The labeled samples are then purified to remove contaminants prior to denaturing and hybridization to a microarray (direct labeling systems) or coupled to the Alexa Fluor™ NHS ester (indirect labeling systems) prior to hybridization (Figure 2).

사양

제품 유형Genomic Labeling System

검출 방법Fluorescence

최종 제품 유형Probes (Labeled DNA)

라벨 또는 염료Cy™3, Cy™5, Alexa Fluor™-Labeled Nucleotides

라벨 또는 염료 포함No

라벨링 타겟Genomic DNA

라벨링 방법Direct Labeling

제품라인BioPrime™

용도(애플리케이션)Chromatin Biology

수량30 Reactions

Unit SizeEach