Thermo Fisher Scientific CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal Antibody (CFTR, 1341)

Applications

• Immunohistochemistry (Paraffin) (IHC (P))
  Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-regulated chloride channel. Approximately 70% of CF cases involve deletion of phenylalanine at position 508 (ΔF508), resulting in abnormal chloride transport. This mutation leads to severe lung and liver disease. Interestingly, CFTR also plays a role in Salmonella typhi infection; ΔF508 heterozygote and homozygote mice showed 86% and 100% reductions in S. typhi intestinal submucosal uptake.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.