Thermo Fisher Scientific TOP2A Polyclonal Antibody
Rabbit polyclonal antibody against human TOP2A for WB and IHC applications. Detects Topoisomerase II alpha involved in DNA synthesis and mitosis. Supplied as liquid, 1 mg/mL, purified by antigen affinity chromatography. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 1415–1509 of Human Topoisomerase II alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2544724 |
Product Specific Information
- Recommended positive controls: 293T, A431, H1299
- Predicted reactivity: Pig (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Topo II alpha plays important roles in DNA synthesis, transcription, and chromosomal segregation during mitosis. It is a sensitive and specific marker of late S-, G2-, and M-phases in transformed and developmentally regulated normal cells.
Topo II alpha is also implicated in drug resistance of tumor cells.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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