Thermo Fisher Scientific TEM5 Polyclonal Antibody
TEM5 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF에 사용 가능. 인간 시료 반응성. Affinity chromatography로 정제된 액상 항체로 PBS+50% glycerol에 보관. 종양 혈관신생 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ADGRA2 (Accession Q96PE1), corresponding to amino acid residues S1276–C1326 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2899953 |
Product Specific Information
Antibody detects endogenous levels of total GPR124.
Target Information
Tumor endothelial markers (TEMs) are significantly up-regulated during angiogenesis and neoangiogenesis, which are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for the development of anti-angiogenic therapies. These include TEM1, TEM5, TEM7, and TEM8.
TEM5 is a member of the adhesion family of G protein-coupled receptors and is localized on the surface of endothelial cells. Unlike TEM1, TEM7, and TEM8, which span the membrane once, TEM5 is a seven-pass transmembrane receptor. It is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Proteolytically processed soluble TEM5 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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