Thermo Fisher Scientific WDYHV1 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:1,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:1,000

Product Specifications

Product Specific Information

• Predicted Reactivity: Rat (88%), Rhesus Monkey (97%), Bovine (83%)
• Store product as a concentrated solution.
• Centrifuge briefly prior to opening the vial.

Target Information

WDYHV1 mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in the N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination, and degradation as specified by the N-end rule.
Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position.
Does not deaminate acetylated N-terminal glutamine.
With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity.
In contrast, a proline at position 2 virtually abolishes deamidation of N-terminal glutamine.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.