Thermo Fisher Scientific Beta-Lactamase (TEM-1) Recombinant Protein

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Molecular weight

38 kDa

Class

Recombinant

Type

Protein

Purity

95% as determined by a Coomassie® blue stained SDS-PAGE gel. Calculated from the protein sequence(s).

Activity

1165 micromoles of penicillin G hydrolyzed per minute per milligram of total protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

200mM potassium phosphate, pH 7, with 50% glycerol

Contains

no preservative

Storage conditions

-80°C

Shipping conditions

Dry ice

Product Specific Information

TEM-1 beta-lactamase is the most widespread enzyme to confer beta-lactam antibiotic resistance to gram-negative bacteria. It is a monomeric protein that requires no metals or cofactors for activity. TEM-1 beta-lactamase efficiently hydrolyzes most penicillin and cephalosporin substrates, including nitrocefin and fluorescent derivates. This protein is intended to be used as a positive control for enzyme-dependent substrate hydrolysis and for use on immunoblots. Other uses include kinetic studies with novel substrates or identification of inhibitors of beta-lactamase as part of an antimicrobial program.

1165 micromoles of penicillin G hydrolyzed per minute per milligram of total
protein.

Target Information

CD248 (CD248 Molecule) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include calcium ion binding and extracellular matrix binding. An important paralog of this gene is CD93. CD248 may play a role in tumor angiogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.