Takara pRI 201 DNA transformation vectors

Overview

• Use pRI 201-AN DNA to transform dicotyledonous plant species
• Use pRI 201-ON DNA to transform monocotyledonous plant species
• Integrate multiple transgenes using a single vector
• High copy number (ColE1 ori) replication in E. coli

More Information

Storage

These vectors should be stored at –20°C and used within 2 years of receipt.

Product citations

Nagaya, S., Kawamura, K., Shinmyo, A. & Kato, K. The HSP terminator of Arabidopsis thaliana increases gene expression in plant cells. Plant Cell Physiol. 51, 328–32 (2010).

Nishiguchi, R., Takanami, M. & Oka, A. Characterization and sequence determination of the replicator region in the hairy-root-inducing plasmid pRiA 4b. MGG Mol. Gen. Genet. 206, 1–8 (1987).

Satoh, J., Kato, K. & Shinmyo, A. The 5`-untranslated region of the tobacco alcohol dehydrogenase gene functions as an effective translational enhancer in plant. J. Biosci. Bioeng. 98, 1–8 (2004).

Sugio, T. et al. Effect of the sequence context of the AUG initiation codon on the rate of translation in dicotyledonous and monocotyledonous plant cells. J. Biosci. Bioeng. 109, 170–3 (2010).

Sugio, T., Satoh, J., Matsuura, H., Shinmyo, A. & Kato, K. The 5`-untranslated region of the Oryza sativa alcohol dehydrogenase gene functions as a translational enhancer in monocotyledonous plant cells. J. Biosci. Bioeng. 105, 300–2 (2008).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.