Thermo Fisher Scientific Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 755
DyLight 755 형광으로 표지된 염소 유래 항-쥐 IgG(H+L) 2차 항체로, WB, IHC, ICC, Flow 등 다양한 면역실험에 적합합니다. 교차흡착 처리로 타종 반응 최소화, 높은 특이성과 감도 제공. 연구용으로 사용됩니다.
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Thermo Fisher Scientific Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 755
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Rat |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Rat IgG-heavy and light chain |
| Conjugate | DyLight™ 755 |
| Excitation/Emission Max | 760/789 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556603 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, human, mouse, rabbit, and sheep |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to bovine, chicken, human, mouse, rabbit, and sheep.
Target Information
Anti-Rat secondary antibodies are affinity-purified with well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of rat IgG targets.
Secondary antibodies increase versatility by enabling use with various detection systems (HRP, AP, fluorescence) and provide greater sensitivity through signal amplification.
Typically, secondary antibodies are produced by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for high specificity.
Available Conjugate Formats
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 647, 680
- AP, APC, Cyanine3, Cyanine5, DSB-X Biotin
- DyLight 488, 550, 594, 650, 680, 800
- FITC, HRP, PE, Texas Red-X, TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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