Thermo Fisher Scientific SEMA6D Monoclonal Antibody (257510)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

257510

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 6D Ser22-His660 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SEMA6D,uniProtId:Q8NFY4-1,ncbiNodeId:9606,antigenRange:22-660,antigenLength:1073,antigenImageFileName:MA5-23955_SEMA6D_Q8NFY4-1_House_mouse.svg,antigenImageFileNamePDP:MA5-23955_SEMA6D_Q8NFY4-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2606529

Product Specific Information

In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Semaphorin 3A and recombinant mouse (rm) Semaphorin 3C is observed and no cross-reactivity with rhSemaphorin 3B, 6A, 6B, 6C, 7A, rmSemaphorin 3E, 3F, or 6D is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

Semaphorins are a large family, including both secreted and membrane associated proteins, many of which have been implicated as inhibitors or chemorepellents in axon pathfinding, fasciculation and branching, and target selection. All semaphorins possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Additional sequence motifs C-terminal to the semaphoring domain allow classification into distinct subfamilies. Results demonstrate that transmembrane semaphorins, like the secreted ones, can act as repulsive axon guidance cues. SEMA6D is a class 6 vertebrate transmembrane semaphorin that demonstrates alternative splicing. Six transcript variants have been identified and expression of the distinct encoded isoforms is thought to be regulated in a tissue- and development-dependent manner.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.