Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (4A12E4)
6x-His 태그 단백질 검출용 마우스 단클론 항체. Western blot, ICC/IF 등 다양한 응용에 적합. 0.5 mg/mL 농도의 액상 형태로 제공되며 Protein A로 정제. His-tag 단백질의 정제 및 검출에 활용 가능.
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Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (4A12E4)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5 - 5 µg/mL | View 30 publications |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 10 publications |
| ELISA | - | View 4 publications |
| Immunoprecipitation (IP) | - | View 3 publications |
| Affinity Purification (AP) | - | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
| SDS-PAGE | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4A12E4 |
| Immunogen | Synthetic peptide containing six His residues (6xHis) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533309 |
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at either the N- or C-terminal regions of recombinant proteins to enable purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins lacking specific antibodies by fusing a known epitope to the target protein. His-tagged proteins can be purified using Ni²⁺-NTA adsorbents due to the strong and selective chelation between histidine clusters and nickel ions.
His tag-specific antibodies facilitate detection and co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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