Thermo Fisher Scientific B4GALT1 Polyclonal Antibody
Thermo Fisher Scientific의 B4GALT1 Polyclonal Antibody는 인간 B4GALT1 단백질을 인식하는 토끼 유래 IgG 항체입니다. Western blot, IHC, ICC 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human B4GALT1. Recombinant protein control fragment (Product #RP-90002). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2638439 |
Product Specific Information
Immunogen sequence:
LPQLVGVSTP LQGGSNSAAA IGQSSGELRT GGARPPPPLG ASSQPRPGGD SSPVVDSGPG PASNLTSVPV PHTTALSLPA CPEESPLLVG PMLIEFNMPV DLELVAKQNP NVKM
Highest antigen sequence identity to the following orthologs:
- Mouse: 71%
- Rat: 73%
Target Information
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins with specificity for UDP-galactose, transferring galactose in a beta1,4 linkage to acceptor sugars such as GlcNAc, Glc, and Xyl. Each beta4GalT has distinct roles in glycoconjugate and saccharide biosynthesis.
As type II membrane proteins, they possess an N-terminal hydrophobic signal sequence that directs localization to the Golgi apparatus and serves as a transmembrane anchor. The beta4GalTs form four groups based on sequence similarity:
- beta4GalT1 & beta4GalT2
- beta4GalT3 & beta4GalT4
- beta4GalT5 & beta4GalT6
- beta4GalT7
B4GALT1 is unique among these genes as it participates in both glycoconjugate and lactose biosynthesis. It adds galactose to N-acetylglucosamine residues on glycoprotein carbohydrate chains and, in lactating mammary tissues, forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose ⇔ UDP + lactose. The two enzymatic forms arise from alternative transcription initiation and post-translational processing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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