Takara SfiI restriction enzyme

SfiI restriction site:

GGCCN NNN | NGGCC
CCGGN | NNN NCCGG

Supplied buffer: M

Effect of DNA methylation: enzyme activity is often affected by dcm methylase

Reaction temperature: 50°C

More Information

• Source: Streptomyces fimbriatus
• Concentration: 4–12 units/µl
• Substrate for unit definition: Ad2 DNA
• Genome DNA analysis: Escherichia coli genomic DNA
• Star activity: cleavage at alternative site(s) occurs in the presence of Mn2+ or DMSO
• Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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