Thermo Fisher Scientific ECSIT Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 50-296 of human ECSIT (NP_001135936.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ECSIT,uniProtId:Q9BQ95-1,ncbiNodeId:9606,antigenRange:50-296,antigenLength:431,antigenImageFileName:PA5-109674_ECSIT_Q9BQ95-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109674_ECSIT_Q9BQ95-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

5.44 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2855085

Product Specific Information

Immunogen sequence: SEQSLVPSPP EPRQRPTKAL VPFEDLFGQA PGGERDKASF LQTVQKFAEH SVRKRGHIDF IYLALRKMRE YGVERDLAVY NQLLNIFPKE VFRPRNIIQR IFVHYPRQQE CGIAVLEQME NHGVMPNKET EFLLIQIFGR KSYPMLKLVR LKLWFPRFMN VNPFPVPRDL PQDPVELAMF GLRHMEPDLS ARVTIYQVPL PKDSTGAADP PQPHIVGSGR DAGGVEPLLP DAAGPGVCEE WLGQLRV

Target Information

Activation of NF-kappa-B as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 to kinases such as IRAK and other intermediates such as TNF receptor associated factor (TRAF)-6. ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis, indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.