Thermo Fisher Scientific ECSIT Polyclonal Antibody
ECSIT 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, WB, ICC/IF, ELISA 등에 사용 가능. 인간, 마우스, 랫트 반응성. 고순도 친화 크로마토그래피 정제, -20°C 보관. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 50–296 of human ECSIT (NP_001135936.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 5.44 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2855085 |
Product Specific Information
Immunogen sequence:
SEQSLVPSPP EPRQRPTKAL VPFEDLFGQA PGGERDKASF LQTVQKFAEH SVRKRGHIDF IYLALRKMRE YGVERDLAVY NQLLNIFPKE VFRPRNIIQR IFVHYPRQQE CGIAVLEQME NHGVMPNKET EFLLIQIFGR KSYPMLKLVR LKLWFPRFMN VNPFPVPRDL PQDPVELAMF GLRHMEPDLS ARVTIYQVPL PKDSTGAADP PQPHIVGSGR DAGGVEPLLP DAAGPGVCEE WLGQLRV
Target Information
Activation of NF-kappa-B as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by adapter molecules such as TRIF, TIRAP, and MyD88 to kinases like IRAK and intermediates such as TRAF6. ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TRAF6 in the TLR pathway. ECSIT is also required for bone morphogenetic protein (BMP) signaling and mesoderm formation during mouse embryogenesis, suggesting cross-talk between TLR/IL-1B and BMP signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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