Thermo Fisher Scientific GRP94/HSP90B1 Recombinant Rabbit Monoclonal Antibody (HSP90B1, 3168R)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Flow Cytometry (Flow)

• Tested Dilution: 1–2 µg/10^6 cells

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
• For formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, then cool at room temperature for 20 min.

Target Information

GRP94 is an 803 amino acid protein belonging to the heat shock protein 90 family. It functions as a molecular chaperone in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulate CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in both innate and acquired immunity, dendritic cell maturation and chemotaxis, antibody production, and cross-priming. GRP94 is a potential marker in breast cancer and acts as a peptide acceptor in the endoplasmic reticulum, assisting in peptide loading of MHC class I molecules. Expression of GRP94 suppresses A23187-induced apoptosis and stabilizes calcium homeostasis. It is expressed in melanoma and liver metastases of colon carcinoma cells, as well as in human gastric carcinoma BGC-823 cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.