Thermo Fisher Scientific MSH2 Recombinant Mouse Monoclonal Antibody (rMSH2, 6548)
Recombinant mouse monoclonal antibody recognizing MSH2 protein. Suitable for Western blot and IHC (paraffin). Reacts with dog, cat, and human samples. Provided unconjugated in liquid form, 200 µg/mL, stored at 4°C in PBS with BSA and sodium azide.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Cat, Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rMSH2, 6548 |
| Immunogen | Recombinant full-length human MSH2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA-fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
MSH2 plays a role in neurogenesis and neuronal migration. It is necessary for proper formation of mitotic spindles and chromosome separation during mitosis, and is essential for cytokinesis and cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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