Thermo Fisher Scientific SKAP2 Polyclonal Antibody
Human SKAP2 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC(P), ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS(40% 글리세롤) 용액 상태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human SKAP2. Recombinant protein control fragment (Product #RP-88612). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.10 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2647327 |
Product Specific Information
Immunogen sequence:
GSDKDKQQKG EFAIDGYSVR MNNTLRKDGK KDCCFEISAP DKRIYQFTAA SPKDAEEWVQ QLKFVLQDME SDIIPEDYDE RGELYDDVDH PLPISNPLTS SQPIDDEIYE ELPEEEEDSA PVKVEEQRKM SQDSVHHTSG D
Highest antigen sequence identity to the following orthologs:
- Mouse: 84%
- Rat: 82%
Target Information
Fyb (Fyn binding protein) and the anchoring proteins SKAP55 and SKAP55-R (SKAP55-related protein) associate with the tyrosine kinase p59fyn. SKAP55 and SKAP55-R bind to Fyb through their SH3 domains and function as substrates for p59Fyn in resting T cells.
SKAP55 contains an N-terminal pleckstrin homology domain and a C-terminal SH3 domain binding motif of adjacent arginine and lysine residues followed by tandem tyrosines (RKxxYxxY).
SKAP55-R, similar in overall structure to SKAP55, contains a coiled-coil N-terminal domain.
SKAP55 associates with SLAP-130, another component of the Fyn complex, which plays a role in the regulation of signaling events initiated by lymphocyte antigen receptors leading up to T cell activation.
The human SKAP55 gene maps to chromosome 17q21.32 and encodes a 359 amino acid protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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