Thermo Fisher Scientific VPS34 Polyclonal Antibody
VPS34 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, ICC/IF, IP에 사용 가능. 인간 반응성, 고순도 항원 친화 크로마토그래피 정제. 세포 신호전달 및 자가포식 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human VPS34 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792721 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (86%), Xenopus laevis (92%), Dog (99%), Pig (98%), Bovine (99%).
- Positive Control: 293T, A431, HeLa, HepG2, U87-MG, SK-N-SH, IMR32, SK-N-AS, unboiled hVps34-transfected 293T cells.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PIK3C3 (hVPS34) is the sole class III member of the PI3 Kinase family. PIK3C3 phosphorylates phosphatidylinositol (PI) to produce phosphatidylinositol 3-phosphate [PI(3)P], which recruits downstream effectors containing FYVE and Phox domains to membranes. PIK3C3 is involved in coupling nutrient signaling to mTOR and also plays a role in autophagy and intracellular trafficking. It is involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, likely in the context of PI3KC3-C2. It also contributes to the transport of lysosomal enzyme precursors to lysosomes and to transport from early to late endosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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