Thermo Fisher Scientific 4-Hydroxy-2-hexenal Monoclonal Antibody (5C11.1)
4-Hydroxy-2-hexenal에 특이적인 mouse monoclonal antibody로 Western blot, ELISA, Dot blot에 사용 가능. 1 mg/mL 농도의 액상 형태이며 PBS/glycerol buffer에 보관. Protein G로 정제되었고 -20°C에서 보관. 연구용으로 적합하며 4-HNE와 약한 교차반응을 보임.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| ELISA | Assay-dependent | |
| Dot Blot (DB) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5C11.1 |
| Immunogen | Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735093 |
Additional Formats
Product Specific Information
A 1:1,000 dilution of MA5-27576 was sufficient for detection of 4-Hydroxy-2-hexenal in 1 µg of 4-Hydroxy-2-hexenal-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Specific for 4-Hydroxy-2-hexenal. Slight cross-reactivity with 4-Hydroxynonenal. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
4-Hydroxynonenal (4-HNE, C9H16O2) is an alpha,beta-unsaturated hydroxyalkenal produced by lipid peroxidation in cells. It is the primary alpha,beta-unsaturated hydroxyalkenal formed during this process.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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