Thermo Fisher Scientific AF9 Polyclonal Antibody
AF9 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF, ChIP 등에 사용 가능. 높은 특이성과 재현성을 제공하며, 인간 AF9 단백질(aa 281–532) 기반 항원으로 제작됨. 연구용으로만 사용.
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Thermo Fisher Scientific AF9 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| ChIP assay (ChIP) | Assay-dependent | - |
| ChIP-sequencing (ChIP-Seq) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 281–532 of Human AF9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.46 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545273 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, THP-1, MLLT3 shRNA-transfected 293T, SEM-K2
- Predicted reactivity: Mouse (98%), Rat (98%), Bovine (97%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The human AF9 gene is one of the most common fusion partner genes with the ALL1 (MLL) gene at 11q23, resulting in t(9;11)(p22;q23) translocation. The AF9 gene spans over 100 kb and includes two patient breakpoint cluster regions (BCRs):
- BCR1 within intron 4 (site A)
- BCR2 spanning introns 7 and 8 (site B)
AF9 contains structural elements such as:
- In vivo DNA topo II cleavage site
- In vitro DNase I hypersensitive site in intron 7 (BCR2)
- Two scaffold associated regions (SARs): SAR1 (intron 4) and SAR2 (exons 5–7)
These regions share structural similarities with the MLL breakpoint cluster region (BCR). A DNA breakage and repair model for nonhomologous recombination between MLL and AF9 has been proposed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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