Takara hiPSC to definitive endoderm differentiation kit

The Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture System contains a complete media set and a ready-to-use coating for the differentiation of human induced pluripotent stem (hiPS) cells into definitive endoderm (DE) cells in monolayer culture. The kit includes the Cellartis DEF-CS 100 Culture System for the maintenance of undifferentiated hiPS cells prior to DE cell differentiation.

The Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture System contains a complete media set and a ready-to-use coating for the differentiation of human induced pluripotent stem (hiPS) cells into definitive endoderm (DE) cells in monolayer culture. The kit includes the Cellartis DEF-CS 100 Culture System for the maintenance of undifferentiated hiPS cells prior to DE cell differentiation. The DE cells obtained with this differentiation kit can be used for further differentiation into cells of endodermal origin, such as hepatocytes and pancreatic endoderm cells. For further differentiation of DE cells into hepatocytes, use the Cellartis Hepatocyte Differentiation Kit (Cat. # Y30050); alternately, start directly from iPS cells using the complete Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. # Y30055).

DE cell differentiation from two separate hiPS cell lines using the Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture Medium shows the expected temporal mRNA expression profiles for endodermal markers CXCR4, FOXA2, SOX17, and SOX7; mesoendodermal marker Brachyury; and pluripotency marker OCT4.

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Overview

• Efficient differentiation—generates >80% SOX17-positive cells
• Robust protocol—successful definitive endoderm (DE) cell generation from more than 25 different hiPS cell lines
• Complete kit—media and ready-to-use coating for a seven-day protocol to reproducibly differentiate hiPS cells into DE cells
• Start with a homogeneous population of hiPS cells—kit includes the Cellartis DEF-CS 100 Culture System to maintain undifferentiated cells prior to the start of DE cell differentiation

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Components

Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture System (Cat. # Y30035) contains:

1. Cellartis Definitive Endoderm Differentiation Kit (Cat. # Y30030; not sold separately):

• Definitive Endoderm Diff Coating — 10 ml
• Definitive Endoderm Diff Day 0 — 18 ml
• Definitive Endoderm Diff Day 1 — 18 ml
• Definitive Endoderm Diff Day 2 — 18 ml
• Definitive Endoderm Diff Day 3 — 25 ml
• Definitive Endoderm Diff Day 4 — 47 ml
• Definitive Endoderm Diff Day 6 — 47 ml

2. Cellartis DEF-CS 100 Culture System (Cat. # Y30020; not sold separately. A larger 500-ml version of this product is sold as Cat. # Y30010.):

• DEF-CS Basal Medium — 100 ml

• DEF-CS COAT-1 (for 100 ml) — 800 µl

• DEF-CS GF-1 (for 100 ml) — 300 µl

• DEF-CS GF-2 (for 100 ml) — 100 µl

• DEF-CS GF-3 (for 100 ml) — 40 µl

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.