Thermo Fisher Scientific WDR4 Monoclonal Antibody (OTI4G1), TrueMAB
WDR4 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI4G1). Western blot, ICC/IF, Flow cytometry에 적합. HEK293T 세포에서 발현된 full-length 인간 WDR4 단백질을 면역원으로 사용. Lyophilized 형태, PBS buffer, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4G1 |
| Immunogen | Full length human recombinant protein of human WDR4 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
WDR4 is required for 7-methylguanosine modification of tRNA and belongs to the WD repeat protein family.
WD repeats are conserved regions (~40 amino acids) that facilitate formation of multiprotein complexes involved in processes such as:
- Cell cycle progression
- Signal transduction
- Apoptosis
- Gene regulation
This protein is excluded as a candidate for nonsyndromic deafness (DFNB10) but remains a candidate for other disorders mapped to 21q22.3 and for Down syndrome phenotypes.
Alternative splicing generates at least three transcript variants encoding the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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