Invitrogen™

ElectroMAX™ DH5α-E Competent Cells

ElectroMAX DH5α-E Competent Cells are derived from the DH5α strain and are suitable for transformation by electroporation. It is challenging자세히 알아보기

ElectroMAX DH5α-E Competent Cells are derived from the DH5α strain and are suitable for transformation by electroporation. It is challenging to achieve high transformation efficiency by electroporation using the standard DH5α strain (chemical transformation is more efficient) thus additional mutations were introduced to create the DH5α-E strain that is able to reach >1 x 1010 efficiency by electroporation with non-saturating amounts of DNA. Using this strain, 10 to 50 times greater colony number may be obtained in a single transformation than using the chemically competent DH5α cells. Therefore DH5α-E competent cells are ideal for challenging applications, such as generation of cDNA libraries or in transformations with limited DNA input.

ElectroMAX DH5α-E Competent Cells share several key genetic properties. They carry the _hsd_R mutation to prevent restriction of foreign DNA and the _end_A1 mutation that increases the yield of plasmid DNA prepared using miniprep protocols. The _lac_ZΔM15 marker provides α-complementation of the ß-galactosidase gene, allowing blue-white screening on agar plates containing X-gal or Bluo-gal. DH5α-E allows efficient transformation of large plasmids and can also serve as a host for M13mp cloning vectors if a lawn of DH5αF′IQ is provided to allow plaque formation. We do not recommended using this strain for direct cloning of methylated genomic DNA.

ElectroMAX DH5α-E Competent Cell features
• Greatly increased plasmid yield and quality due to end_A1 mutation
• High-efficiency transformation with plasmids 30 kb in size
• Blue/white screening of recombinant clones due to _lac_ZΔM15
• Ensured insert stability due to _rec_A1 mutation
• _hsdR for efficient transformation of unmethylated DNA from PCR applications
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Note:** A high-voltage electroporation apparatus is required.

Genotype
F– φ80_lac_ZΔM15 Δ(lac_ZYA-_arg_F)U169 _rec_A1 _end_A1 _hsd_R17(rK–, mK+) _gal _pho_A _sup_E44 λ-_thi_-1 _gyr_A96 _rel_A1
Genetic marker descriptions

Find the strain and format that fits your needs
DH strains are available in chemically competent and electrocompetent cell formats.
Explore Multishot format for high throughput applications.
MAX Efficiency DH5αF´IQ Competent Cells are available in chemically competent format.
Explore bacterial growth media formats.

사양

제품 유형Electrocompetent Cells

에프에피솜 포함No

플라스미드 품질 개선Yes (endA1)

메틸화 DNA 클로닝No

형질전환 효율 수준High Efficiency (>1 x 109 cfu/μg)

항생제 내성 박테리아No

불안정 DNA 클로닝Not suitable for cloning unstable DNA

블루/화이트 스크리닝Yes (lacZΔM15)

고처리량 호환성Low

플라스미드May be used for plasmids >20 kb

비메틸화 DNA 준비No

재조합 감소Yes (recA1)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)No

종E. coli (K12)

형식Tube

제품라인ElectroMAX™

수량5 x 100 μL

Unit SizeEach