Thermo Fisher Scientific CD95 (APO-1/Fas) Monoclonal Antibody (SM1/23), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent

View 3 publications 3 publications

Functional Assay (Functional)

Assay-Dependent

-

Inhibition Assays (IA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

SM1/23

Immunogen

Rec. Human CD95 (APO-1/Fas)

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• FITC
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2-7.4

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10596808

Product Specific Information

Description: BMS140 is suitable as primary antibody for staining in FACS analysis.

BMS140FI can be used for direct staining of cells. BMS140BT can be used in a two-step procedure using the biotin-(strept-)avidin system.

Functional studies:

BMS140 was found to block induction of apoptosis induced by clone SM1/1 or other commercial anti APO-1 antibodies.

Applications Tested: Flow Cytometry, Functional Studies (Blocking).

Purity: >95%.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.