Thermo Fisher Scientific BRF1 Polyclonal Antibody
BRF1 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, Western blot 및 Immunocytochemistry에 적합합니다. 인간 BRF1 단백질에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- PA565686
- 판매단위
- pk
카탈로그
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Applications
Western Blot (WB)
- Tested Dilution: 0.04–0.4 µg/mL
Immunocytochemistry (ICC/IF)
- Tested Dilution: 0.25–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human TFIIIB90 (BRF1), recombinant protein control fragment (Product #RP-104935) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS (pH 7.2) with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping conditions | Wet ice |
| RRID | AB_2664311 |
Product Specific Information
Immunogen sequence:
PSYTAGQRKLR MKQLEQVLSK KLEEVEGEIS SYQDAIEIEL ENSRPKAKGG LASLAKDGST EDTASSLCGE EDTEDEELEA AASHLNKDL
- Highest antigen sequence identity to orthologs: mouse 88%, rat 87%
Target Information
RNA polymerase III synthesizes tRNA, 5s rRNA, 7SL RNA, and U6 snRNA. It is overexpressed in many transformed cell lines and tumors, as cells must duplicate its protein components before division.
To maintain rapid growth, cells produce high levels of Pol III transcribed RNA, requiring TFIIIB and TFIIIC2 transcription factor complexes.
TFIIIC2 consists of five subunits (TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90, TFIIIC63) that are overexpressed in malignant cells, including ovarian carcinomas.
TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be key in deregulation of malignant cells.
The TFIIIB complex includes TBP, TFIIB-related factor 1 (TFIIIB90, BRF1), and TFIIIB, which are upregulated in transformed cells.
In many carcinomas, tumor suppressors RB and p53 are inactivated, affecting their ability to bind and inactivate TFIIIB.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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