Thermo Fisher Scientific BrdU Monoclonal Antibody (OTI7H4), TrueMAB
BrdU 검출용 단클론 항체로 세포 증식 연구에 적합. IHC 및 ICC/IF 적용 가능. 마우스 IgG2a 형식의 비결합 항체. 동결건조 형태로 제공되며, 1 mg/mL 농도. DNA 복제 세포의 식별에 활용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7H4 |
| Immunogen | Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882) to ensure carrier-free conditions.
Target Information
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside analog of thymidine used to detect proliferating cells.
It incorporates into newly synthesized DNA during the S phase, substituting for thymidine.
BrdU-specific antibodies can detect incorporated BrdU, identifying cells actively replicating DNA.
Antibody binding requires DNA denaturation, typically by acid or heat treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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