Thermo Fisher Scientific SBP2 Polyclonal Antibody
Thermo Fisher Scientific의 SBP2 Polyclonal Antibody는 인간, 마우스, 랫트 시료에서 SECISBP2 단백질을 검출하는 토끼 유래 폴리클로날 항체입니다. Western blot 및 IHC(P) 실험에 적합하며, 고순도 친화 크로마토그래피 정제 제품입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human SECISBP2 (Accession Q96T21), corresponding to amino acid residues V680–N730 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2854015 |
Product Specific Information
Antibody detects endogenous levels of total SECISBP2.
Target Information
Eukaryotes require a selenocysteine (Sec) insertion sequence (SECIS) element in the 3′ untranslated region of the mRNA to decode the UGA codon as Sec.
SECIS-binding protein 2 (SBP-2) specifically binds selenoprotein mRNAs to form a functional complex and is essential for the insertion of Sec into selenoproteins.
Purified SBP-2 interacts specifically with the SECIS element in the phospholipid hydroperoxide glutathione peroxidase mRNA. SBP-2 shows binding activity in the liver and testis as well as hepatoma cells.
SBP-2 binds to a conserved RNA binding domain shared with several ribosomal proteins and eukaryotic translation termination release factor 1.
A second domain located N-terminal to the RNA binding domain required for Sec insertion allows SBP-2 to stably associate with the ribosomal fraction of cells.
SBP-2 preferentially stimulates incorporation directed by the selenoprotein P and phospholipid hydroperoxide glutathione peroxidase SECIS elements.
SBP-2 may have a distinct function in selecting the ribosomes for Sec insertion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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