Thermo Fisher Scientific UGGT1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

5-20 µg/mL

-

Immunocytochemistry (ICC/IF)

5-20 µg/mL

-

Product Specifications

Species Reactivity

Human, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human UGGT1 protein, Leu1221-Leu1536 (Accession #Q9NYU2) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:UGGT1,uniProtId:Q9NYU2-1,ncbiNodeId:9606,antigenRange:1221-1536,antigenLength:1555,antigenImageFileName:PA5-145405_UGGT1_Q9NYU2-1_Rabbit.svg,antigenImageFileNamePDP:PA5-145405_UGGT1_Q9NYU2-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.05% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3091396

Target Information

UDP-glucose:glycoprotein glucosyltransferase (UGGT) is a 170 kDa lumenal endoplasmic reticulum (ER) resident protein that catalyzes monoglucosylation of high mannose oligosaccharides. UGGT catalyzes the transfer of glucose from UDP-glucose to Man7-9GlcNAc2-moiety of unfolded glycoproteins in a Ca2+ dependent reaction. UGGT binds unfolded glycoprotein via two elements on its substrate: a N-acetylglucosamine unit on the oligosaccharide and hydrophobic amino acids that are exposed in the denatured conformation. It is postulated that UGGT serves as a sensor that is able to distinguish between folded and denatured protein in the ER.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.