Thermo Fisher Scientific NEK3 Polyclonal Antibody
Human NEK3 단백질에 특이적인 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF 실험에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며 PBS/glycerol buffer에 보관됩니다. 연구용으로 세포주기 관련 NEK3 단백질 분석에 활용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human NEK3. Recombinant protein control fragment (Product #RP-99574). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.7 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2644605 |
Product Specific Information
Immunogen sequence:
RGGSVIKYSK NTTRKQWLKE TPDTLLNILK NADLSLAFQT YTIYRPGSEG FLKGPLSEET EASDSVDGGH DSVILDPERL EPGLDEED
Highest antigen sequence identity to the following orthologs:
- Mouse: 75%
- Rat: 74%
Target Information
NIMA was originally shown in Aspergillus nidulans to be necessary for entry into mitosis. NIMA-related mammalian proteins have since been identified as Nek1, Nek2, Nek3, and Nek4 (also designated STK2 or NRK2).
High expression of Nek1 is seen in male and female germ cell lines of mouse. Nek2 is the closest known mammalian relative to NIMA, and its expression peaks at the G2 to M phase transition.
Nek3 is a predominantly cytoplasmic enzyme detectable in all organs studied. Levels of Nek3 remain unchanged throughout the cell cycle but are elevated in G0-arrested, quiescent fibroblasts. In developing testicular germ cells, differential expression patterns of Nek1, Nek2, and Nek4 indicate possible overlapping but distinct functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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