Thermo Fisher Scientific CD53 (TSPAN25) Monoclonal Antibody (161-2)
CD53(TSPAN25)에 특이적인 마우스 단클론 항체로, 인간 시료에 반응합니다. ICC/IF, Flow Cytometry, Functional Assay에 사용 가능하며, 비결합형 액상 형태로 제공됩니다. 단백질 A/G로 정제되었으며, PBS(pH 7.4)에 보존제 없이 -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Functional Assay (Functional) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 161-2 |
| Immunogen | Stimulated human leukocytes |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Baboon or Horse.
Target Information
CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage.
This molecule forms complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4, or tetraspanins CD37, CD81, and CD82, modulating various signaling processes.
CD53 is involved in radioresistance of tumor cells and has an anti-apoptotic effect when triggered.
In the thymus, CD53 is up-regulated in response to positive selection signals during T cell development and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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