Thermo Fisher Scientific CENP-F/Mitosin Polyclonal Antibody
CENP-F/Mitosin 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, ICC/IF, IP에 적합합니다. 인간 시료 반응성이 있으며, 항원 친화 크로마토그래피로 정제된 액상 제품입니다. 세포주기 연구 및 염색체 분리 관련 연구에 활용됩니다.
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Applications
Western Blot (WB)
- Assay-dependent
Immunocytochemistry (ICC/IF)
- Dilution: 1:500–1:2,000
Immunoprecipitation (IP)
- 2–5 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1800 and 1850 of human centromere protein F (Mitosin) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For ICC, acetone fixation is recommended
Target Information
This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2, it associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and intracellular bridge in late anaphase and telophase, respectively, and is subsequently degraded.
Its localization suggests a role in chromosome segregation during mitosis. It may form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft-versus-host disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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