Invitrogen™

One Shot™ TOP10 Electrocomp™ E. coli

One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency자세히 알아보기

One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 1010 cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids. TOP10 Electrocomp E. coli cells are transformed via electroporation and achieve higher transformation efficiencies than chemically competent cells. They can therefore be used in more challenging applications, including cloning low amounts of DNA or cDNA and gDNA library construction.

TOP10 E. coli cells are genetically similar to the DH10B strain but have been reported to be more resilient to stress conditions like osmotic shock and acidic pH stress. These cells carry mutations in the methylation-dependent restriction system (mcr_A, _mcr_BC, and _mrr) allowing them to clone both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes. Similar to other DH strains, TOP10 has the _lac_ZΔM15 genotype, providing for the option of blue-white screening on plates containing either X-Gal or Bluo-Gal.

One Shot TOP10 Electrocomp E. coli cells offer:
• Transformation efficiencies of >1 x 1010 cfu/μg
• _hsd_R for efficient transformation of unmethylated DNA from PCR amplifications
• _mcr_A for efficient transformation of methylated DNA from genomic preparations
• _lac_ZΔM15 for blue/white color screening of recombinant clones
• _end_A1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
• _rec_A1 for reduced occurrence of nonspecific recombination in cloned DNA

Easy-to-use One Shot format
TOP10 Chemically Competent E. coli cells are supplied in the convenient, single-reaction One Shot format. The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, thereby helping save time and prevent contamination.

Genotype
F–mcr_A Δ(_mrr_-_hsd_RMS-_mcr_BC) φ80_lac_ZΔM15 Δ_lac_X74 _rec_A1 _ara_D139 Δ(_ara-leu)7697 _gal_U _gal_K λ–_rps_L(StrR) _end_A1 _nup_G

Find the strain and format that fit your needs
We offer other DH strains in chemically competent and electrocompetent cell formats.
Electroporation cuvettes with 0.1 cm, 0.2 cm, and 0.4 cm gaps are available.
The TOP10 strain is available in several MultiShot formats for high throughput applications.
Explore bacterial growth media formats.

Note: TOP10 cells do not require IPTG to induce expression from the lac promoter. A high-voltage electroporation apparatus is required.

사양

제품 유형Electrocompetent Cells

에프에피솜 포함No

플라스미드 품질 개선Yes (endA1)

메틸화 DNA 클로닝Yes (mcrA)

형질전환 효율 수준High Efficiency (>1 x 1010 cfu/μg)

항생제 내성 박테리아No

불안정 DNA 클로닝No

블루/화이트 스크리닝Yes (lacZΔM15)

고처리량 호환성Low

플라스미드May be used for plasmids >20 kb

비메틸화 DNA 준비No

재조합 감소Yes (recA13)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)Yes (Streptomycin)

종E. coli (K12)

형식Tube

제품라인One Shot™

수량11 x 50 μL

Unit SizeEach