Invitrogen™

ReadyProbes™ Cell Viability Imaging Kit, Blue/Red

ReadyProbes® Cell Viability Imaging Kit (Blue/Red) is a ready-to-use kit that can quickly and easily determine the viability of cells.자세히 알아보기

ReadyProbes® Cell Viability Imaging Kit (Blue/Red) is a ready-to-use kit that can quickly and easily determine the viability of cells. Just add 2 drops each of room temperature, stable NucBlue® Live reagent (Hoechst 33342) and propidium iodide to 1 mL of cell growth media, then determine viability by counting total vs dead cells. NucBlue® Live reagent stains the nuclei of all the cells and can be detected using a standard DAPI filter. Propidium iodide stains only the nuclei of cells with compromised plasma membrane integrity and is detected with a standard TRITC/RFP (orange) filter set. This kit is appropriate for fluorescence microscopy, fluorescence micro plate readers, and flow cytometry.

NucBlue® Live reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 360/460 nm)
Propidium iodide: stains the nuclei of dead cells with compromised plasma membrane; detected with standard TRITC/RFP (orange) filter set (excitation/emission maxima: 535/617 nm)
See other ReadyProbes® reagents for cell staining
Learn more about other assays for cell viability

Suggestions for use
• NucBlue® Live reagent and propidium iodide can be added directly to cells in full growth media or a compatible buffer solution.
• In most cases, 2 drops/mL and an incubation time of 5 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.

사양

세포 유형Mammalian Cells, Eukaryotic Cells

색상Blue, Red

설명ReadyProbes Cell Viability Imaging Kit, Blue/Red

검출 방법Fluorescence

용도(애플리케이션)Viability Assay

용도 (장비)Fluorescence Microscope

제품 유형Cell Viability Imaging Kit

염료 유형3 x 2.5 mL NucBlue™ Live; 3 x 2.5 mL propidium iodide

방출460, 617 nm

여기 파장 범위360, 535 nm

형식Dropper bottles

제품라인ReadyProbes

수량1 kit

배송 조건Room Temperature

Unit SizeEach