Thermo Fisher Scientific IL-2 Monoclonal Antibody (MQ1-17H12), Brilliant Ultra Violet 395, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532
• APC
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and major immunoregulatory cytokine.
This antibody is non-neutralizing and has been tested for intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
Use at 5 µL (0.5 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test).

Fluorochrome Information:
Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is designed for cytometers equipped with a 355 nm UV laser. Ensure your instrument supports this fluorochrome.

Staining Panel Recommendation:
When using multiple polymer dye-conjugated antibodies (e.g., Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• or Brilliant Stain Buffer™ (Product # 00-4409-75))
  to minimize non-specific interactions.

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine crucial for proliferation of activated T cells, differentiation of B cells, NK cells, monocytes, and macrophages.
IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric complex sharing its gamma chain with IL-4 and IL-7.
Disruption of IL-2-related genes in mice induces colitis-like disease, indicating its essential role in immune regulation.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

제품 이미지