Gibco™

MCDB 131 Medium, no glutamine

MCDB 131 Medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular자세히 알아보기

MCDB 131 Medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular endothelial cells (HMVEC). MCDB 131 Medium is also used with other cell types, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells.

This MCDB 131 Medium is manufactured as follows:

With	Without
• Phenol Red	• L-glutamine
• Sodium pyruvate	• HEPES

The complete formulation is available.

Using MCDB 131
MCDB 131 Medium contains many components not found in traditional basal media, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins. These additions allow the medium to be supplemented with very low levels of serum or defined components. MCDB 131 Medium contains no proteins or growth factors, and is often supplemented with EGF, hydrocortisone, glutamine, and low levels of Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell type. MCDB 131 Medium uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Dual-site cGMP manufacturing
For supply chain continuity, we manufacture MCDB 131 at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

사양

세포 유형Human Microvascular Endothelial Cells (HMVEC), Human Omental Microvascular Cells, Hepatocytes, Myocytes, Smooth Muscle Cells

농도1 X

Culture TypeMammalian Cell Culture

형태Liquid

제품 유형MCDB 131 Medium

유통 기한12 Months From Date of Manufacture

멸균Sterile-filtered

첨가제 포함Low Glucose, Phenol Red, Sodium Pyruvate

첨가제 없음No Glutamine, No HEPES

제품라인Gibco™

수량500 mL

배송 조건Room Temperature

Unit SizeEach