Thermo Fisher Scientific Gata-3 Monoclonal Antibody (TWAJ), Brilliant Violet 786, eBioscience
Gata-3 전사인자를 인식하는 TWAJ 단클론 항체로, 인간·마우스·돼지·원숭이에 반응합니다. Brilliant Violet 786 형광체 결합으로 유세포분석(Flow Cytometry)에 최적화되어 있습니다. Th2 분화 및 T세포 발달 연구용으로 적합하며, 광민감성이 있으므로 암조건에서 보관하십시오.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Gata-3 Monoclonal Antibody (TWAJ), Brilliant Violet 786, eBioscience
Applications
- Flow Cytometry (Flow): 5 µL (0.5 µg)/test
Species Reactivity
- Human
- Mouse
- Pig
- Rhesus monkey
Host / Isotype
- Rat / IgG2b, kappa
Recommended Isotype Control
Rat IgG2b kappa Isotype Control (eB149/10H5), Brilliant Violet™ 786, eBioscience™
Product Specifications
| 항목 | 내용 |
|---|---|
| Class | Monoclonal |
| Type | Antibody |
| Clone | TWAJ |
| Conjugate | Brilliant Violet™ 786 |
| Excitation/Emission Max | 406/788 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- Brilliant UV 395
- Brilliant UV 563
- Brilliant UV 661
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 711
- eFluor 450
- eFluor 660
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a transcription factor essential for thymic development and Th2 differentiation. Gata-3 expression is observed in the CNS, skin, mammary glands, and kidney during embryonic development, and is critical for T-cell commitment and survival. In the thymus, expression occurs mainly in CD4 single-positive cells.
Alternative splice variants (50 and 45 kDa) are recognized by this antibody.
Staining requires the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
Cross-reactivity in rhesus monkeys has been reported.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-diluted and tested for intracellular staining of mouse thymocytes using Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00). Recommended usage: 5 µL (0.5 µg)/test.
Brilliant Violet™ 786 (BV786) emits at 786 nm and is compatible with cytometers equipped with a 405 nm laser. Use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) when combining multiple polymer dye-conjugated antibodies to minimize non-specific interactions.
Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
Note: BV786 is not compatible with methanol-based fixation.
Excitation: 407 nm; Emission: 786 nm; Laser: Violet Laser.
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen.
Target Information
GATA3 is a transcription factor that binds to enhancer elements of T-cell receptor (TCR) genes (alpha, beta, gamma, delta) and regulates their expression. It contains a zinc finger domain and is abundantly expressed in T-lymphocytes, playing a crucial role in TCR gene activation. The human gene spans 17 kb with six exons, encoding two zinc fingers highly conserved with GATA1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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