Thermo Fisher Scientific GCN5 Monoclonal Antibody (S.775.4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200-1:800

View 1 publication 1 publication

Immunoprecipitation (IP)

1:200

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Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

S.775.4

Immunogen

Synthetic peptide corresponding to the amino terminus of human GCN5L2 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GCN5,uniProtId:Q92830-1,ncbiNodeId:9606,antigenRange:1,antigenLength:837,antigenImageFileName:MA5-14884_GCN5_Q92830-1_Rabbit.svg,antigenImageFileNamePDP:MA5-14884_GCN5_Q92830-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

134 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10978625

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with the related PCAF protein.

Target Information

SAGA (Spt-Ada-Gen5 histone acetyltransferase complex) is a histoneacetylase complex which has Gcn5p as catalyst subunit and functions overlapping with the fundamental transcription factor TFIID which has Taf1p as catalyst subunit. However, SAGA and TFIID have different allotment and each accomplishes the important role in the transcription for Housekeeping gene group and Stress Responding gene group. Also, SAGA is a gigantic protein complex which is composed of Ada protein group (5 kinds), TBP related protein group (4 kinds), TAF protein group (5 kinds that also pertain to TFIID), and other protein groups (>6 kinds). As its molecular function, there is the chemical modification of histone, or the recruitment by direct interaction of transcription regulating factor on DNA, or the control of transcription starting reaction by TBP. Gcn5p that show histoneacetylase activity is one kind of the above-mentioned Ada protein group, and in the case of budding yeast it is composed of 439 amino acid residues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.