Thermo Fisher Scientific Human VEGF-165 Recombinant Protein

Thermo Fisher Scientific Human VEGF-165 Recombinant Protein

Applications and Tested Dilution

Product Specifications

Product Specific Information

Mature human VEGF165 was expressed in Pichia pastoris (strain KM71). GenBank Accession #003376, amino acids 27–191.
For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: EGGGQNHHEV.
Purity is >95% by SDS-PAGE analyses.

Activity: The biological activity of recombinant human VEGF165 was determined by measuring the proliferation of human umbilical vein endothelial cells (HUVEC).

Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.

Reconstitution and Dilution:
Store lyophilized material at 4°C. Reconstitute in 1 mL of distilled or deionized water.
Dilute in a buffer containing carrier protein, aliquot, and store at or below -70°C, avoiding multiple freeze-thaw cycles.
PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.

Target Information

The vascular endothelial growth factor (VEGF) family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF.
VEGF and its receptor system are fundamental regulators of angiogenesis signaling.
Most tumors require angiogenesis, and VEGF is a potent angiogenic cytokine linked to this process.
Five different isoforms of VEGF have been described, generated by alternative splicing from a single VEGF gene.
These isoforms bind to two tyrosine-kinase receptors, flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), primarily expressed on endothelial cells.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.